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If you are having 3 test sample solution, having DNA, RNA and Protein respectively. How will you differentiate between these samples that which one is what?

HII,
the sample can be subjected to AGE or PAGE.on AGE the DNA gets sepaerated depanding on various factors you will have to read the principle.
On PAGE the protein gets seperated
nowadays there are a lot many equipments whican can isolate all the three together.i will send you a link of an experiment you shall be clear once you go through it


http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/RNA%20and%20mRNA/UM_TotalDNARNAProtein.pdf

Any further doubts definetly do let me know

dna is the short form of deoxy ribo nucleic acid and is responsible for the transfer of genetic character from one generation to another .
RNA is the short form of ribo nucleic acid and is the component of protein .
and protein is the building element which consists dna and rna. so these three are totally different.

by doing southern,northern and western blot tests.these r tests used to identify dna,rna and protien respectively

DNA contains the deoxy form at the two prime end of ribose that is reason we called as DEOXY RIBONUCLEIC ACID and also it is truly a hereditary material to transfer the genetic information. In RNA contains oxy form at the two prime end of ribose that is reason we called as OXY RIBONUCLEIC ACID and it contains the uracil as a nitrogen base instead of thymine in DNA. But protein is expressive form of a gene and used for the growth of the individual. Protein is a complex of aminoacids.

Originally Posted by Unregistered

If you are having 3 test sample solution, having DNA, RNA and Protein respectively. How will you differentiate between these samples that which one is what?

Phyically its really tough to differentiate between all the three solution but it can be tested only on the basis of chemical properties as proties have sulphur coat and DNA and RNA are the two different sugars

DNA deoxyribonueclic acid....contains genetic information needed for development of living organisms....
RNA RIBONUECLIC ACID........consists of long chain of nuecleotides found in the nuecleus.......
PROTEINS are organic compounds made of amino acids.........

As i have no knowledge about science still I am trying my best yo reply tour answer as I think so that you are a Guest who deliberately is taking our TEST as you HIMSELF know the answer-
transcription and translation can differentiate the DNA,RNA & protein respectively.A protein produced by mutated gene may be harmful within the living conditions of an organism.Am I RIGHT?

Originally Posted by Unregistered

If you are having 3 test sample solution, having DNA, RNA and Protein respectively. How will you differentiate between these samples that which one is what?

to differentiate between all the three sample sollution you judt need to carry out the northern bloting test, southern blotting test and western blotting test.
This the best method to differentite between them all

for the differentiation b/w DNA, RNA, Protein u perform a test. take a small amount of a sample to it add dil. HNO3,if the solution turns pale yellow then it is the protein, for the differetiation b/w DNA & RNA u have to go for the "gel electrophoresis" , DNA will move fast while RNA will move with a bit slow speed and DNA consist of thymine while RNA consist of uracil. if u r provided with 3 samples then first perform the test for protein it'll help u..

Protein can b differentiated form nucleic acids by comparing the absorbance at 260nm and at 280nm.. Protein will show maximum absorbance at 280nm...and to differentiate between rna and dna we can use schiff's test. schiff's test is positve only when aldehyde group is present.. on acid hydrolysis both rna and dna loose a purine residue.. leaving behind the sugar residue.. in case of deoxyribose a free aldehyde group is present so will give a positve schiff's test .. but since rna does not hav a aldehyde group it will show negative schiff's test

hii

to differentiate between DNA, RNA and Protein
DNA contains the deoxy form at the two prime end of ribose that is reason we called as DEOXY RIBONUCLEIC ACID and also it is truly a hereditary material to transfer the genetic information. In RNA contains oxy form at the two prime end of ribose that is reason we called as OXY RIBONUCLEIC ACID and it contains the uracil as a nitrogen base instead of thymine in DNA. But protein is expressive form of a gene and used for the growth of the individual. Protein is a complex of aminoacids
nowadays there are a lot many equipments whican can isolate all the three together.i will send you a link of an experiment you shall be clear once you go through it


http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/RNA%20and%20mRNA/UM_TotalDNARNAProtein.pdf


good luck

Differentiating DNA, RNA & Proteins :

1. Blotting Techniques:
Western Blot to detect proteins
Southern Blot to detect dna
Northern Blot to detect rna

2. Following isolation procedures:
In case of blotting techniques, it will be time-consuming. One can easily follow standard isolation protocol to isolate & identify whether the sample consist of dna/rna or protein.

Here, the samples are allowed to follow isolation procedures & at the end their absorbance is measured in UV-spectrophotometer. For, dna, protein & rna, they gets absorbed at a specific wavelength. So, the reading will help us differentiate between dna, rna & protein.

Dear,

How to differentiate between DNA, RNA and Protein are as follows: -

1. DNA - DNA means Deoxyribonucleic Acid. DNA is located in the cell nucleus.
DNA is a double helix formed by base pairs attached to a sugar-phosphate backbone.

2. RNA - RNA means Ribonucleic Acid. RNA is a biologically type of molecule that consists of a long chain of nucleotide units. RNA consisting of a long, usually single-stranded chain of alternating phosphate.

3. Protein - Proteins are biochemical compounds consisting of one or more polypeptides.


Good Luck

DNA : Deoxyribonucleic acid
DNA is the hereditary material in humans and almost all other organisms.

RNA:Ribonucleic acid
RNA is a biologically important type of molecule that consists of a long chain of nucleotide units.

PROTIENS:
Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form in a biologically functional way.

Colour estimation for the specific sugar for dna and rna.

positive biuret test will differentiate proteins from nucleic acids..

Originally Posted by Unregistered

DNA contains the deoxy form at the two prime end of ribose that is reason we called as DEOXY RIBONUCLEIC ACID and also it is truly a hereditary material to transfer the genetic information. In RNA contains oxy form at the two prime end of ribose that is reason we called as OXY RIBONUCLEIC ACID and it contains the uracil as a nitrogen base instead of thymine in DNA. But protein is expressive form of a gene and used for the growth of the individual. Protein is a complex of aminoacids.

uracil takes the place of adenine, not thymine