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Sir I want few previous question papers of biotechnology.

Hello,

||==Previous years question papers for Biotechnology==||

1. The term cistorn, muton and recon were introduced by
(A) Watson and Crick
(B) S. Benzer
(C) Meselson
(D) Morgan
Answer: (B)
2. Extranuclear genetic material is found in
(A) Plastid and nucleus
(B) Mitochondria and plastids
(C) Nucleus and cytoplasm
(D) Mitochondria and nucleus
Answer: (B)

3. The molecular formulae of deoxyribose sugar and ribose sugar respectively are
(A) C5 H10 O4 and C5H10O6
(B) C5 H10 O4 and C5H10O5
(C) C5 H10 O5 and C5H10O4
(D) C5 H10 O5 and C6H10O4
Answer: (B)
4. The nitrogen bases which pair with two hydrogen bonds are
(A) Adenine and thymine
(B) Adenine and Cytosine
(C) Cytosine and guanine
(D) Cytosine and adenine
Answer: (A)
5. DNA differs from RNA in
(A) Presence of deoxyribose sugar
(B) Presence of thymine base
(C) Property of replication
(D) All the above
Answer: (D)
6. DNA molecules makes a complete turn after every
(A) 20 Å
(B) 34 Å
(B) 3.4 Å
(D) 10 base pairs
Answer: (D)
7. The distance between two successive nitrogenous base pairs is
(A) 34 Å
(B) 36 Å
(C) 20 Å
(D) 3.4 Å
Answer: (D)
8. In nucleoside, nitrogen base is attached to pentose sugar at
(A) Carbon – 1 of pentose sugar
(B) Carbon – 2 of pentose sugar
(C) Carbon – 4 of pentose sugar
(D) Carbon – 5 of pentose sugar
Answer: (A)
9. If the strand of DNA has 35 nucleotide how many phosphodiester bonds would exist
(A) 34
(B) 35
(C) 24
(D) 70
Answer: (A)
10. In eukaryotic DNA replication, lagging strand is formed by
(A) RNA fragments
(B) Okazaki fragments
(C) DNA fragments
(D) Nucleotide fragments
Answer: (B)
11. The enzyme DNA polymerase can work only in
(A) 3¢ ® 5¢ direction
(B) 5¢ ® 3¢ direction
(C) Both the direction
(D) 5¢ ® 5¢ direction
Answer: (B)
12. Enzyme required for removing RNA primer during DNA replication is
(A) DNA primase
(B) DNA ligase
(C) DNA polymerase I
(D) DNA polymerase III
Answer: (C)
13. During DNA replication, the reunion or recoiling of separated DNA strand is prevented by
(A) Helix destabilizing protein
(B) Single strnad binding protein
(C) Rep protein
(D) Both (A) and (B)
Answer: (D)
14. The enzyme that cuts the bonds of DNA molecule at the origin of replication is
(A) Endonuclease
(B) DNA polymerase
(C) DNA gyrase
(D) DNA ligase
Answer: (A)
15. Which of the following enzyme is required to release the tension imposed by uncoiling of strands?
(A) Endonuclease
(B) DNA ligase
(C) DNA gyrase
(D) DNA helicase
Answer: (C)
16. The cellular composition of m-RNA is
(A) 5-10%
(B) 3-5%
(C) 10-20 %
(D) 70-80%
Answer: (B)
17. Formation of mRNA from DNA is called
(A) Transformation
(B) Transduction
(C) Traslation
(D) Transcription
Answer: (D)
18. The ratio of purines and pyrimidines in mRNA is not 1:1 because the nitrogenous bases are
(A) Unpaired
(B) Paired
(C) Paired only in loops
(D) Paired in stems
Answer: (A)
19. The codons which may present at 3¢ end of mRNA
(A) UAA
(B) UAG
(C) UGA
(D) Any one of these
Answer: (D)
20. Which of the following is not tool of genetic engineering?
(A) Vectors
(B) Enzymes
(C) Foreign DNA
(D) GMO
Answer: (D)
21. In recombinant DNA technology a plasmid vector is cleaved by
(A) Modified DNA ligase
(B) A heated alkaline solution
(C) The same enzyme that cleave the donor DNA
(D) The different enzyme other than that cleave the donor DNA
Answer: (C)
22. The most common plasmid vector used in genetic engineering is
(A) PBR 328
(B) PBR 322
(C) PBR 325
(D) PBR 330
Answer: (B)
23. ‘Nif gene’ for nitrogen fixation is cereal crops like wheat, jowar etc. is introduced by cloning
(A) Rhizobium meliloti
(B) Bacillus thuringiensis
(C) Rhizopus
(D) Rhizophora
Answer: (A)
24. Eco RI is an
(A) Ligase
(B) Polymerase
(C) Restriction enzyme
(D) Gyrase
Answer: (C)
25. The transgenic plant flavr savr tomato carries an artificial gene for
(A) Delay ripening process
(B) Longer shell life
(C) Added flavours
(D) All of these
Answer: (D)

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1. Hirudin is obtained from the transgenic plant
(A) Brassica napus
(B) Hibiscus rosasinesis
(C) Raphanus sativus
(D) Vinca rosea
Answer: (A)
2. Bt Cotton is
(A) Cloned plant
(B) Transgenic plant
(C) Hybrid plant
(D) Mutated plant
Answer: (B)
3. Dolly sheep was genetically similar to
(A) The mother from which nucleated fertilized egg was taken
(B) The mother from which nuclear DNA of udder cell was taken
(C) The surrogate mother
(D) Both surrogate mother and nuclear donor mother
Answer: (B)
4. Genome is
(A) Genes on nuclear DNA
(B) Nuclear DNA + mitochondrial DNA
(C) Nuclear DNA + chloroplast DNA
(D) Nuclear DNA + Mitochondrial DNA + Chloroplast DNA
Answer: (D)
5. A technique of using very small metal particles coated with desired gene in the gene transfer is called
(A) Electroporation
(B) Microinjection
(C) Liposome
(D) Biolistics
Answer: (D)
6. The complete set of chromosomal and extrachromosomal genes of an organisms is called
(A) Genome
(B) Gene pool
(C) Gene bank
(D) Gene library
Answer: (A)
7. The study of all the proteins coded by the genome is called
(A) Proteome
(B) Proteomics
(C) Genome
(D) Protein formation
Answer: (B)
8. Sequencing of genomic DNA is included under
(A) Structural genomics
(B) Functional genomics
(C) Proteomics
(D) Transgenesis
Answer: (A)
9. Gene expression, regulation and phenotype production are studied in second phase of genome analysis called
(A) Structural genomics
(B) Functional genomics
(C) Proteomics
(D) Transmeiosis
Answer: (B)
10. A flowering plant lily have ______ more DNA than humans
(A) 10 times
(B) 15 times
(C) 18 times
(D) 13 times
Answer: (C)
11. In forensic science which of the following is used?
(A) Bacterial cloning
(B) DNA foot printing
(C) DNA fingerprinting
(D) DNA cloning
Answer: (C)
12. DNA fingerprinting is based on
(A) Occurance of VNTR’s
(B) Knowledge of human karyotype
(C) Cloned DNA
(D) Recombinant DNA
Answer: (A)
13. VNTRs represnets-
(A) New terminal regions in DNA
(B) Functional genes in the DNA
(C) Split genes in the sample DNA
(D) Specific non-coding sequences with unique tandem repeats
Answer: (D)
14. Which ones produce androgenic haploids in anther cultures?
(A) Anther wall
(B) Tapetal layer of anther wall
(C) Connective tissue
(D) Young pollengrains
Answer: (D)
15. Variations observed during tissue culture of some plants are known as
(A) Clonal variations
(B) Somatic variations
(C) Somaclonal variations
(D) Tissue culture variations
Answer: (C)
16. Virus free plants can be obtained through
(A) Anitibiotic treatment
(B) Bordeaux micture
(C) Root tip culture
(D) Shoot tip culture
Answer: (D)
17. To raising of plants from a small tissue in culture is known as
(A) Macroproduction
(B) Micropropagation
(C) Tissue culture
(D) Mass production
Answer: (B)
18. Callus is
(A) Tissue that forms embryo
(B) an insoluble carbohydrate
(C) Unorganised actively dividing mass of cells maintained in culture
(D) Tissue that growth to form embryoid
Answer: (C)
19. Biopatents are ______.
(A) Right to use invention
(B) Right to use biological entities
(C) Right to use products
(D) Right to use process
Answer: (B)
20. African plant Pentadiplandra is used as______.
(A) Low calories sweetner
(B) 2000 times sweeter agent
(C) Sweetner for diabetic patients
(D) All of these
Answer: (D)
21. Which organism was used as bioweapon derived from______.
(A) Clostridium
(B) Yerstsinia pestis
(C) Fusarium species
(D) Green algae
Answer: (C)
22. A set standards used to regulate own or community activity in relation to biological world is
(A) Biopotency
(B) Biopiracy
(C) Biowar
(D) Bioethics
Answer: (D)
23. Biopiracy means
(A) Use of biopatents
(B) Thefts of plants and animals
(C) Stealing of bioresources
(D) Exploitation of bioresources without authentic permission
Answer: (D)
24. Bioethcs is related to
(A) Preventing biopiracy
(B) Regulation of unethical activities likegene cloning in animals
(C) Preventing theft of living materials
(D) Moral guidance to the problems in biology
Answer: (B)
25. Three dimensional shape of tRNA is
(A) L-shaped
(B) Clover leaf-like
(C) X-shaped
(D) Y-shaped
Answer: (B)