#1  
27th May 2012, 10:34 PM
mohankonisi
 
Join Date: May 2012
Location: vizinagaram
Posts: 1

What is the principle involved in IR Spectrophotometry


what is priciple involved in the I.R SPECTROPHOTOMETRY




  #2  
9th September 2012, 06:14 PM
lisajones
 
Join Date: Sep 2012
Posts: 1
Default Re: What is the principle involved in IR Spectrophotometry

IR radiation does not have enough energy to induce electronic transitions as seen with UV. Absorption of IR is restricted to compounds with small energy differences in the possible vibrational and rotational states.For a molecule to absorb IR, the vibrations or rotations within a molecule must cause a net change in the dipole moment of the molecule. The alternating electrical field of the radiation (remember that electromagnetic radiation consists of an oscillating electrical field and an oscillating magnetic field, perpendicular to each other) interacts with fluctuations in the dipole moment of the molecule. If the frequency of the radiation matches the vibrational frequency of the molecule then radiation will be absorbed, causing a change in the amplitude of molecular vibration.
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